Monday, June 6 |
ARRIVAL AND REGISTRATION |
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| Tuesday, June 7 |
PGD for Single Gene Disorders by Polar Body (PB) Biopsy |
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| 09.00 - 09.45 |
Opening and Introductory Lecture: Advantages and disadvantageous of PB diagnosis |
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| 09.45 - 10.00 |
Participants will be divided into 3 groups, up to 5 in each |
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| 10.00 - 12.30 |
Preparation for micromanipulation, First PB (PB1) removal
Second PB (PB2) removal
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| 12.30 - 14:00 |
LUNCH AND DISCUSSION |
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| 14.00 - 18.00 |
Simultaneous workshops: Each group performs the demonstrated techniques under supervision of the moderator |
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| Wednesday, June 8 |
PGD for Chromosomal Disorders by PB Biopsy |
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| 09.00 - 10.00 |
State of the art lecture: Accuracy of PB testing for chromosomal abnormalities by FISH and microarray technology |
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| 10.00 - 12.30 |
Simultaneous removal of PB1 and PB2
Each participant performs the demonstrated technique under supervision of the moderator |
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| 12.30 - 14:00 |
LUNCH AND DISCUSSION |
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| 14.00 - 18.00 |
Preparation, spreading and fixation of PB1 and PB2
Hybridization in situ with different probes
Analysis and scoring fluorescent signals in PB1 and PB2
24-Chromosome testing by microarray technology
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| Thursday, June 9 |
Interpreting FISH and PCR results of PB analysis |
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| 09.00 - 12.00 |
Analysis and scoring of FISH results
Analysis and interpretation of PCR results
and 24-Chromosome testing by microarray technology |
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| 12.30 - 14:00 |
LUNCH AND DISCUSSION |
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| 14.00 - 17.00 |
Simultaneous workshops: Each group performs the demonstrated techniques under supervision of the moderator
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| 17.00 - 18:00 |
GENERAL DISCUSSION & CLOSING REMARKS |